Pdf micropropagation of karanj pongamia pinnata pierre. Micropropagation definition and meaning collins english. Quizlet flashcards, activities and games help you improve your grades. It is a fast growing nonfood legume, capable of symbiotic biological nitrogen fixation bnf, a process absent in other more well established biodiesel feedstocks e. It is done under sterile conditions in a laboratory using plant seed or tissue. Evaluation of antibacterial activity of pongamia pinnata l. Usually meristem or buds without callus reduced somoclonal variation axillary branching. It is found mainly in the native western ghats in india, northern australia, fiji and in some regions of eastern asia.
Micropropagation propagation in vitro is pres ently the most important commercial application of plant tissue culture. By using micropropagation the rate of increase can be speeded up by as much as ten times. Pierrea native indian biodiesel tree from cotyledonary node article pdf available january 2010 with 740 reads. In case of dioecious species, where one of the sex is more desirable then under such circumstances plants of desired sex can be selectively multiplied by this technique. Employing plant tissue culture techniques for micropropagation and further. Multiplication of genetically identical copies of a cultivar by asexual reproduction is called clonal propagation. Micropropagationomicsgroupjournal of biotechnology and.
Shoot culture 9most widely used method for commercial micropropagation 9relatively high genetic stability in the plants produced shoot culture advantages 9reliable rates and consistency of shoot multiplication 93 8 fold multiplication rate per month 9preexisting meristems are least susceptible to genetic changes micropropagation stages stage 0. Knudson, l, 1922 nonsymbiotic germination of orchid seeds, botanical gazette lxxiii. Mar, 2017 micropropagation of facifrora edulis slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Transesterification of crude pongamia oil using koh. Pelagia research library advances in applied science research, 2014, 51. Pdf murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of. Chapter2 micropropagation and large scale culture of c. Micropropagation in horticulture, by s gunn the plantsman new series vol. Multiple shoot bud induction and plant regeneration studies of. It is often known by the synonym pongamia pinnata as it was moved to the genus millettia only recently. Pongamia pinnata pongam environmental horticulture university. Because methanolic bark extract of pongamia pinnata has shown good. Micropropagation is the only viable method of multiplying genetically modified cells or cells after protoplast fusion.
Plant tissue culture offers new ways for the improvement of this crop after many years of recalcitrance. Advantages of micropropagation requires relatively small growing area gives propagules such as minitubersmicrocorms for plant multiplication throughout the year. Information and translations of micropropagation in the most comprehensive dictionary definitions resource on the web. Lewis knudson, 1922 1st in vitro embryo germination orchid ie the invention of in vitro embryo culture. Principles of tissue cult ure and micropropagation. With orchids it is the only way to reproduce the plants as. Micropropagation 264 study guide by mberlitz includes 65 questions covering vocabulary, terms and more.
Meristem micropropagation of cassava manihot esculenta. Research article physico chemical analysis of pongamia. Micropropagation of passiflora edulis from leaf explant 7. Murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of multiple shoots from maturetreederived axillary meristems of pongamia pinnata. Aug 18, 2007 murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of multiple shoots from maturetreederived axillary meristems of pongamia pinnata. Oct 16, 2007 a complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. It is the fact that micropropagation is the only commercially viable method of clonal propagation of most of the horticultural crops. Preparation of biodiesel from crude oil of pongamia pinnata. Plant propagation by tissue culture is known as micropropagation. A complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings.
Enhancing rate of multiplication shoot multiplication short cycle 26 weeks small size propagules storage on large scale, easily transported. Micropropagation of mature pongamia pinnata pierre. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. Micropropagation is the truetotype propagation of a selected genotype using in vitro culture techniques. Derris indica bennet fabaceae pongam, indian beech. Study the effect of growth regulators on micropropagation of gloriosa superba l. Micropropagation definition of micropropagation by the.
Pappilonaceae is a nonedible oil of indian origin lakshmikanthan, 1978. The purpose of stagei is to initiate axenic culture. Micropropagation is not always the perfect means of multiplying plants. Learn vocabulary, terms, and more with flashcards, games, and other study tools.
Plant cells for micropropagation may be newly transformed as a result of genetic engineering, or they might be cells taken from plant tissue. Pdf in vitro regeneration of pongamia pinnata pierre. Multiplication of genetically identical copies of a cultivar by asexual reproduction using tissue culture techniques iv requirements. Micropropagation enables growers to increase the production of plants that normally propagate very slowly such as narcissus and other bulbous crops. Research article physico chemical analysis of pongamia pinnata. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of diseasefree plants and its merits and demerits. The micropropagation of chestnut via axillary shoots involves four stages.
General physical properties of pongamia pinnata oil blends b10, b20 and diesel parameters pongamia pinnata diesel b20b10 ph 3. Pongamia pinnata is a legume plant which has great potential to be used as a. Micropropagation is a combination of the arts and sciences of plant multiplication in vitroand plant acclimitization. It is very expensive, and can have a labor cost of more than 70%. Micropropagation is labour intensive involving manual handling at 3 or 4 stages.
An external file that holds a picture, illustration, etc. Micropropagation differs from all other conventional propagation methods in that aseptic conditions are essential to achieve success. Vegetative propagation of sterile hybrids can be used as parent plants for seed production. This stage begins with the excision of meristem tissue from an identified stock plant. Millettia pinnata is a species of tree in the pea family, fabaceae, native in tropical and temperate asia including parts of indian subcontinent, china, japan, malaysia, australia and pacific islands. Successful in vitro propagation depends on the specific requirements and precise manipulation of various factors such as the type of explants used, physiological state of the mother plant. In vitro micropropagated samples were obtained from the tissue culture facility of the mikocheni agriculture research institute mari dar es salaam, tanzania and were propagated as described by konan et al.
The labour intensive nature of micropropagation has led to outsourcing of micropropagation from european nurseries to contractors in low wage, typically asian, countries, with the plantlets being returned for growing on. Effects of pongamia pinnata on lipid peroxidation products and antioxidants in hyperammonemic rats with reference to circadian variations were evaluated by essa and subramanian 29. Sep 10, 2014 aseptic method of clonal propagation is called as micropropagation and it offer the advantage of large number of truetotype plantlets can be produced with relatively short time and space from a single individual. Typically, this involves culturing excised meristematic tissue on a special medium that encourages axillary bud development. Locations where specimens of millettia pinnata have been collected and identified by state herbaria red circles represent collection locations labelled as pongamia pinnata and green circles collections labelled as millettia pinnata avh 2010. Micropropagation of pongamia pinnata a sustainable biofuel plant species for semi arid climatic conditions vinod kumar patil and naik, g. Micropropagation definition of micropropagation by the free. Explant from 60 day old plant central part of the leafs sterilization with ethanol,sodium hypochlorite, tween 20 explants collection and sterilization. A tissue culture technique for plant propagation in which tissue is taken from a plant and grown in a laboratory to produce plantlets that are. One such genus is that of trilliums, and while i do not intend to work on trilliums exclusively, i do intend to concentrate on this group. Pongamia is native to southern and southeast asia, as well as northern australia. Prospect of the legume tree pongamia pinnata as a clean. Micropropagation definition of micropropagation at. The in vivo clonal propagation is often difficult, expensive and even unsuccessful.
Pdf micropropagation of mature pongamia pinnata pierre. Plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. Gavino rotor, 1949 1st in vitro vegetative propagation. A piece of plant tissue called an explant is a cut from the plant, b disinfested removal of surface contaminants, and c. Literature survey of pongamia pinnata revealed its potential being like hepatoprotective and antioxidant 30. To 10 g of crude pongamia oil, a known amount of catalyst koh, 0. A monoculture is produced after micropropagation, leading to a lack of overall disease resilience, as all progeny plants may be vulnerable to the same infections.
Preparation of biodiesel from crude oil of pongamia. Micropropagation definition, the propagation of plants from tissue cultures, often from single cells. The new shoots are then separated and cultured, and the cycle is repeated until finally the shoots are transferred to a medium that promotes root development, to produce plantlets. Sprouting of buds was 64% on medium devoid of plant growth regulators pgr. Introduction of disease free new cultivars is possible through micropropagation 6. Farming system indian council of agricultural research. Curcuma longa is an indian spice derived from the rhizomes of the plant and has a long history of use in ayurvedic medicine as a treatment for inflammatory conditions. The process of micropropagation can be divided into four stages. Direct adventitious shoot bud formation on hypocotyls explants in. Development of micropropagation protocols would allow commercial tissue culture companies to mass propagate and make available to the nursery trade elite lines of selected ornamental, herbaceous perennials. Pongamia pinnata is a fastgrowing, mediumsized, evergreen or briefly deciduous, glabrous shrub or tree with a broad crown of spreading or drooping branches 303 title.
The rhs libraries can, for a fee, provide photocopies, by post if necessary. It is the truetotype propagation of a genotype that comprises many stepsstock plant care, explant selection and sterilization, media manipulation to obtain proliferation, rooting, acclimation, and growing on of liners and is. Incorporation of ba, kn, or z was ineffective in enhancing sprouting frequency or. Of the various culture media that have been assayed for micropropagation of. Lewis knudson, 1922 1st in vitro embryo germination orchid ie the invention of in vitro embryo culture knudson, l, 1922 nonsymbiotic germination of orchid seeds, botanical gazette lxxiii. Axillary buds, biodiesel plant, leaf, micropropagation, pongamia, plant growth regulators, plant tissue culture. Micropropagation of pongamia pinnata through enhanced. Micropropagation offers the potential to produce thousands or even millions of plants per annum. Micropropagation of ornamental perennials university of. Pongamia pinnata oil with conventional diesel with b10 and b20 proportions are furnished in the table3. Department of biotechnology, gulbarga university, gulbarga585106, karnataka, india corresponding author email.
Micropropagation is an important tool for rapid multiplication and the creation of genetic variability in african violets saintpaulia ionantha wendl. Micropropagation of mature pongamia pinnata pierre springerlink. Pdf pongamia pinnata pierre is a tree legume, having potential in. Unit ii biotechnology and plants lesson 7 plant tissue culture 169 cells and tissue the starting point for micropropagation can be individual cells or small amounts of plant tissue.
Micropropagation of african violet saintpaulia ionantha. Application of tissue culture techniques for genetic up gradation of economically important plants has been reported. If you continue browsing the site, you agree to the use of cookies on this website. Milletia pinnata syn pongamia pinnata, locally known as karanja. Multiple shoots were induced in vitro from nodal segments through forced axillary branching. Transesterification of crude oil of pongamia pinnata 2. Micropropagation of orchids micropropagation is the process used to replicate plants such as orchids, the word micropropagation means simply propagation in miniature. Regeneration of pongamia through tissue culture from immature cotyledons a through. Antimalarial efficacy of pongamia pinnata l pierre against. Pongamia pinnata is a fastgrowing, mediumsized, evergreen or briefly deciduous, glabrous shrub or tree with a broad crown of spreading or drooping branches. Murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for. A monoculture is produced after micropropagation, leading to a lack of overall disease resilience, as all progeny plants may be vulnerable to the same infections an infected. It is also used to provide a sufficient number of plantlets for planting from a. Introduction to micropropagation cornell university.
History of plant tissue culture propagation at cornell. Recent studies2 4 revealed that most chlorophyllous plants in vitro have the ability to grow photoautotrophically, and that the low co 2 concentration in the airtight. The plant extract contains flavonoids, carbohydrates, glycosides, international journal of chemtech. Micropropagation is an advanced vegetative propagation technology for producing a large number of genetically superior and pathogenfree transplants in a limited time and space. The main characteristic of this technique is rapid clonal multiplication which results in the production of a large number of uniform plants from a limited number of mother stock plants.
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